UOSD SAFU

Foto (piccola)

Maurizio Fanciulli

Area Medicina Molecolare

 Research Projects

The mission of the SAFU UOSD focuses on the establishment of innovative mouse models of human cancer including implantation of tumor specimens into immunocompromised mice at the heterotopic and orthotopic sites and genetically engineered mouse models. All mouse models are devoted to study cancer initiation, immune system roles, tumor angiogenesis, environmental carcinogenesis, invasion as well as response to novel anticancer strategy. Currently, several models are being designed to allow in vivo imaging of tumor development from earlier stages and to follow tumor response to therapeutics. Besides to the research activities, this UOSD has the responsibility for day-to-day management of the Institute animal house. In agreement, this structure coordinates the activity of Animal Welfare Body (D.Lgs. n.26/2014), evaluating scientific projects in which are involved animal experimentations. 

 Publications

 

A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP.

Pagan JK, Arnold J, Hanchard KJ, Kumar R, Bruno T, Jones MJ, Richard DJ, Forrest A, Spurdle A, Verdin E, Crossley M, Fanciulli M, Chenevix-Trench G, Young DB, Khanna KK.
J Biol Chem. 2007 May 18;282(20):15248-57. Epub 2007 Mar 22.
 
Che-1: a new effector of checkpoints signaling.
Floridi A, Fanciulli M.
Cell Cycle. 2007 Apr 1;6(7):804-6. Epub 2007 Apr 22. Review.
 
NRAGE associates with the anti-apoptotic factor Che-1 and regulates its degradation to induce cell death.
Di Certo MG, Corbi N, Bruno T, Iezzi S, De Nicola F, Desantis A, Ciotti MT, Mattei E, Floridi A, Fanciulli M, Passananti C.
J Cell Sci. 2007 Jun 1;120(Pt 11):1852-8. Epub 2007 May 8.
 
Blandino G, Fanciulli M, Levrero M, Piaggio G.
Cell Death Differ. 2007 Jul;14(7):1390-1. Epub 2007 May 18.
 
The anti-apoptotic factor Che-1/AATF links transcriptional regulation, cell cycle control, and DNA damage response.
Passananti C, Fanciulli M.
Cell Div. 2007 Jul 16;2:21.
 
Utrophin up-regulation by an artificial transcription factor in transgenic mice.
Mattei E, Corbi N, Di Certo MG, Strimpakos G, Severini C, Onori A, Desantis A, Libri V, Buontempo S, Floridi A, Fanciulli M, Baban D, Davies KE, Passananti C.
PLoS One. 2007 Aug 22;2(8):e774.
 
Che-1/AATF, a multivalent adaptor connecting transcriptional regulation, checkpoint control, and apoptosis.
Passananti C, Floridi A, Fanciulli M.
Biochem Cell Biol. 2007 Aug;85(4):477-83. Review.
 
Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex.
Buontempo S, Barbato C, Bruno T, Corbi N, Ciotti MT, Floridi A, Fanciulli M, Passananti C.
Neuroreport. 2008 Mar 26;19(5):531-5. doi: 10.1097/WNR.0b013e3282f85c1b.
 
Novel activation domain derived from Che-1 cofactor coupled with the artificial protein Jazz drives utrophin upregulation.
Desantis A, Onori A, Di Certo MG, Mattei E, Fanciulli M, Passananti C, Corbi N.
Neuromuscul Disord. 2009 Feb;19(2):158-62. doi: 10.1016/j.nmd.2008.11.005. Epub 2009 Jan 21.
 
Nuclear HBx binds the HBV minichromosome and modifies the epigenetic regulation of cccDNA function.
Belloni L, Pollicino T, De Nicola F, Guerrieri F, Raffa G, Fanciulli M, Raimondo G, Levrero M.
Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19975-9. doi: 10.1073/pnas.0908365106. Epub 2009 Nov 11.
 
Corbi N, Batassa EM, Pisani C, Onori A, Di Certo MG, Strimpakos G, Fanciulli M, Mattei E, Passananti C.
PLoS One. 2010 Dec 31;5(12):e14481. doi: 10.1371/journal.pone.0014481
 
 
 
Targeting the MDM2/MDM4 interaction interface as a promising approach for p53 reactivation therapy.
Pellegrino M, Mancini F, Lucà R, Coletti A, Giacchè N, Manni I, Arisi I, Florenzano F, Teveroni E, Buttarelli M, Fici L, Brandi R, Bruno T, Fanciulli M, D'Onofrio M, Piaggio G, Pellicciari R, Pontecorvi A, Marine JC, Macchiarulo A, Moretti F.
Cancer Res. 2015 Nov 1;75(21):4560-72. doi: 10.1158/0008-5472.CAN-15-0439. Epub 2015 Sep 10.
 
Application of RNA-Seq Technology in Cancer Chemoprevention.
Goeman F, Fanciulli M.
Methods Mol Biol. 2016;1379:31-43. doi: 10.1007/978-1-4939-3191-0_4.
 
Discovering Che-1/AATF: a new attractive target for cancer therapy.
Iezzi S, Fanciulli M.
Front Genet. 2015 Apr 10;6:141. doi: 10.3389/fgene.2015.00141. eCollection 2015. Review.
 
The prolyl isomerase Pin1 affects Che-1 stability in response to apoptotic DNA damage.
De Nicola F, Bruno T, Iezzi S, Di Padova M, Floridi A, Passananti C, Del Sal G, Fanciulli M.
J Biol Chem. 2007 Jul 6;282(27):19685-91. Epub 2007 Apr 27.
 
Che-1 activates XIAP expression in response to DNA damage. Bruno T, Iezzi S, De Nicola F, Di Padova M, Desantis A, Scarsella M, Di Certo MG, Leonetti C, Floridi A, Passananti C, Fanciulli M.Cell Death Differ. 2008 Mar;15(3):515-20. Epub 2007 Nov 30.
 
Che-1 promotes tumor cell survival by sustaining mutant p53 transcription and inhibiting DNA damage response activation. Bruno T, Desantis A, Bossi G, Di Agostino S, Sorino C, De Nicola F, Iezzi S, Franchitto A, Benassi B, Galanti S, La Rosa F, Floridi A, Bellacosa A, Passananti C, Blandino G, Fanciulli M. Cancer Cell. 2010 Aug 9;18(2):122-34. doi: 10.1016/j.ccr.2010.05.027
 
Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair. Cortellino S, Xu J, Sannai M, Moore R, Caretti E, Cigliano A, Le Coz M, Devarajan K, Wessels A, Soprano D, Abramowitz LK, Bartolomei MS, Rambow F, Bassi MR, Bruno T, Fanciulli M, Renner C, Klein-Szanto AJ, Matsumoto Y, Kobi D, Davidson I, Alberti C, Larue L, Bellacosa A. Cell. 2011 Jul 8;146(1):67-79. doi: 10.1016/j.cell.2011.06.020. Epub 2011 Jun 30.
 
Centrosomal Che-1 protein is involved in the regulation of mitosis and DNA damage response by mediating pericentrin (PCNT)-dependent Chk1 protein localization. Sorino C, Bruno T, Desantis A, Di Certo MG, Iezzi S, De Nicola F, Catena V, Floridi A, Chessa L, Passananti C, Cundari E, Fanciulli M.J Biol Chem. 2013 Aug 9;288(32):23348-57. doi: 10.1074/jbc.M113.465302. Epub 2013 Jun 24.
 
HIPK2 sustains apoptotic response by phosphorylating Che-1/AATF and promoting its degradation. De Nicola F, Catena V, Rinaldo C, Bruno T, Iezzi S, Sorino C, Desantis A, Camerini S, Crescenzi M, Floridi A, Passananti C, Soddu S, Fanciulli M. Cell Death Dis. 2014 Sep 11;5: e1414. doi: 10.1038/cddis.2014.381.
 
Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53.
Desantis A, Bruno T, Catena V, De Nicola F, Goeman F, Iezzi S, Sorino C, Gentileschi MP, Germoni S, Monteleone V, Pellegrino M, Kann M, De Meo PD, Pallocca M, Höpker K, Moretti F, Mattei E, Reinhardt HC, Floridi A, Passananti C, Benzing T, Blandino G, Fanciulli M. Cell Death Dis. 2015 May 21;6: e1764. doi: 10.1038/cddis.2015.117.
 
Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy.
Desantis A, Bruno T, Catena V, De Nicola F, Goeman F, Iezzi S, Sorino C, Ponzoni M, Bossi G, Federico V, La Rosa F, Ricciardi MR, Lesma E, De Meo PD, Castrignanò T, Petrucci MT, Pisani F, Chesi M, Bergsagel PL, Floridi A, Tonon G, Passananti C, Blandino G, Fanciulli M. EMBO J. 2015 May 5;34(9):1214-30. doi: 10.15252/embj.201489920. Epub 2015 Mar 14
 
Che-1/AATF: A Critical Cofactor for Both Wild-Type- and Mutant-p53 Proteins.
Bruno T, Iezzi S, Fanciulli M.
Front Oncol. 2016 Feb 15;6:34. doi: 10.3389/fonc.2016.00034. eCollection 2016. Review
 
Multitargeting activity of miR-24 inhibits long-term melatonin anticancer effects.
Mori F, Ferraiuolo M, Santoro R, Sacconi A, Goeman F, Pallocca M, Pulito C, Korita E, Fanciulli M, Muti P, Blandino G, Strano S.
Oncotarget. 2016 Apr 12;7(15):20532-48. doi: 10.18632/oncotarget.7978
 
UCN-01 enhances cytotoxicity of irinotecan in colorectal cancer stem-like cells by impairing DNA damage response.
Signore M, Buccarelli M, Pilozzi E, De Luca G, Cappellari M, Fanciulli M, Goeman F, Melucci E, Biffoni M, Ricci-Vitiani L.
Oncotarget. 2016 Jul 12;7(28):44113-44128. doi: 10.18632/oncotarget.9859.
 
AATF: A new actor in the complex world of mTOR.
Fanciulli M.
Mol Cell Oncol. 2015 Jul 15;3(1):e1040946. doi: 10.1080/23723556.2015.1040946. eCollection 2016 Jan.
PMID: 27308559 Free PMC Article
 
Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells.
Catena V, Bruno T, De Nicola F, Goeman F, Pallocca M, Iezzi S, Sorino C, Cigliana G, Floridi A, Blandino G, Fanciulli M.
Oncotarget. 2016 Oct 25;7(43):70546-70558. doi: 10.18632/oncotarget.12060.
 
Deptor: not only a mTOR inhibitor.
Catena V, Fanciulli M.
J Exp Clin Cancer Res. 2017 Jan 13;36(1):12. doi: 10.1186/s13046-016-0484-y. Review.
 
Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1α stabilization.
Bruno T, Valerio M, Casadei L, De Nicola F, Goeman F, Pallocca M, Catena V, Iezzi S, Sorino C, Desantis A, Manetti C, Blandino G, Floridi A, Fanciulli M.
J Exp Clin Cancer Res. 2017 Feb 18;36(1):32. doi: 10.1186/s13046-017-0497-1
 
CHK1-targeted therapy to deplete DNA replication-stressed, p53-deficient, hyperdiploid colorectal cancer stem cells.
Manic G, Signore M, Sistigu A, Russo G, Corradi F, Siteni S, Musella M, Vitale S, De Angelis ML, Pallocca M, Amoreo CA, Sperati F, Di Franco S, Barresi S, Policicchio E, De Luca G, De Nicola F, Mottolese M, Zeuner A, Fanciulli M, Stassi G, Maugeri-Saccà M, Baiocchi M, Tartaglia M, Vitale I, De Maria R.
Gut. 2017 Apr 7. pii: gutjnl-2016-312623. doi: 10.1136/gutjnl-2016-312623. [Epub ahead of print]
PMID: 28389531
 
DNA damage repair and survival outcomes in advanced gastric cancer patients treated with first-line chemotherapy.
Ronchetti L, Melucci E, De Nicola F, Goeman F, Casini B, Sperati F, Pallocca M, Terrenato I, Pizzuti L, Vici P, Sergi D, Di Lauro L, Amoreo CA, Gallo E, Diodoro MG, Pescarmona E, Vitale I, Barba M, Buglioni S, Mottolese M, Fanciulli M, De Maria R, Maugeri-Saccà M.
Int J Cancer. 2017 Jun 1;140(11):2587-2595. doi: 10.1002/ijc.30668. Epub 2017 Mar 11.
 
Metformin-induced ablation of microRNA 21-5p releases Sestrin-1 and CAB39L antitumoral activities.
Pulito C, Mori F, Sacconi A, Goeman F, Ferraiuolo M, Pasanisi P, Campagnoli C, Berrino F, Fanciulli M, Ford RJ, Levrero M, Pediconi N, Ciuffreda L, Milella M, Steinberg GR, Cioce M, Muti P, Strano S, Blandino G.
Cell Discov. 2017 Jul 4;3:17022. doi: 10.1038/celldisc.2017.22. eCollection 2017.

Staff

Maurizio Fanciulli      (PhD, Staff Scientist)     06/5266-2800

Federico De Marco    (MD PhD, Staff Scientist)       06/5266-2794
Carlo Leonetti            (PhD, Staff Scientist)           06/5266-2534
Giulia Piaggio             (PhD, Staff Scientist)           06/5266-2458
Giancarlo Cortese       (Staff Technician)               06/5050348
Tiziana Bruno             (Staff Technician)               06/5266-2578
Enrico Spugnini          (Veterinary)
Aymone Gurtner,       (Project Leader)
Gabriele Toietta,         (Project Leader)
Francesca De Nicola (Senior Scientist)
Simona Iezzi               (Senior Scientist)
Isabella Manni            (Senior Scientist)
Silvia Baldari              (Post-Doc)
Valeria Catena            (Post-Doc)
Lucia Cicchillitti         (Post-Doc)
Matteo Pallocca          (Post-Doc)
Manuela Porru            (Post-Doc)
Cristina Sorino           (Post-Doc)
Emmanuela Falcone   (PhD student)
Luca Pompili              (PhD Student)
Susanna Falcucci        (Graduate Student)
Umberto Brocco         (Graduate Student)
Bruno Amadio           (Technician)
Adele Petricca            (Administrative collaborator)